5UCC
Crystal structure of the ENTH domain of ENT2 from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 88.901, 88.901, 67.259 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.420 - 1.830 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.18460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gzc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 1.185 | |
| Rpim | 0.527 | |
| Number of reflections | 17422 | |
| <I/σ(I)> | 41.76 | 2.04 |
| Completeness [%] | 99.3 | 97.6 |
| Redundancy | 6.5 | 5.7 |
| CC(1/2) | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.2 M sodium chloride, 0.1M phosphate-citrate pH 4.2, 20% (w/v) PEG8K, 0.3 M NDSB195 |
