5UC8

Crystal structure of human Heme Oxygenase-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 21 21 21
Unit cell lengths	77.760, 84.619, 139.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.783 - 2.000
R-factor	0.1976
Rwork	0.196
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q32
RMSD bond length	0.007
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.072	0.037	0.492
Rmeas	0.084
Rpim	0.044
Total number of observations	237797
Number of reflections	65740
<I/σ(I)>	16.6
Completeness [%]	99.8	98.1	100
Redundancy	3.6	3.4	3.6
CC(1/2)		0.997	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Bis-Tris (pH 6.5) and 24% (w/v) PEG 2,000 monomethyl ether