5UBO
Mical-oxidized Actin complex with Gelsolin Segment 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.200, 69.250, 179.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.590 - 2.390 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1eqy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.590 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.092 | 1.711 |
| Number of reflections | 28998 | |
| <I/σ(I)> | 5.7 | 2.27 |
| Completeness [%] | 99.7 | 96.4 |
| Redundancy | 38.1 | 33.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | PEG 6000, NaCl, imidazole, ATP, calcium |
