5UB8
Crystal structure of YPT31, a Rab family GTPase from Candida albicans, in complex with GDP and Zn(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.303, 43.392, 41.662 |
| Unit cell angles | 90.00, 89.05, 90.00 |
Refinement procedure
| Resolution | 29.572 - 2.350 |
| R-factor | 0.1893 |
| Rwork | 0.185 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cpj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.586 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.165 | 0.786 |
| Number of reflections | 8864 | |
| <I/σ(I)> | 13.13 | 2.56 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 15% (w/v) PEG3350, 25 mM zinc acetate |
