5UAC

Escherichia coli RNA polymerase and Rifampin complex, wild-type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.62990
Spacegroup name	P 21 21 21
Unit cell lengths	188.958, 204.430, 313.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.982 - 3.800
R-factor	0.2491
Rwork	0.248
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yg2
RMSD bond length	0.017
RMSD bond angle	1.564
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.800
Number of reflections	119630
<I/σ(I)>	9.8
Completeness [%]	100.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT