5UA2
Mycobacterium tuberculosis KstR in complex with a 26-bp DNA operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.365, 79.545, 77.124 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 46.751 - 2.900 |
| R-factor | 0.2312 |
| Rwork | 0.230 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cxg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.760 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.185 | 0.803 |
| Number of reflections | 15213 | |
| <I/σ(I)> | 5 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.981 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 20% PEG 600, 0.2 M imidazole malate |
