5U9O
Cocrystal structure of the intermembrane space region of the plastid division proteins PARC6 and PDV1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.227, 80.192, 84.070 |
| Unit cell angles | 88.36, 80.99, 83.49 |
Refinement procedure
| Resolution | 38.234 - 3.370 |
| R-factor | 0.2535 |
| Rwork | 0.250 |
| R-free | 0.31810 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.500 |
| High resolution limit [Å] | 3.370 | 7.000 | 3.370 |
| Rmerge | 0.287 | 0.096 | 0.982 |
| Rmeas | 0.322 | ||
| Rpim | 0.194 | ||
| Total number of observations | 69814 | ||
| Number of reflections | 22865 | ||
| <I/σ(I)> | 3.6 | ||
| Completeness [%] | 97.1 | 99 | 87 |
| Redundancy | 3.1 | 3.4 | 2.5 |
| CC(1/2) | 0.985 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 12% polyethylene glycol (PEG) 8000, 160 mM ammonium sulfate and 0.8% glycerol |
