5U9E
Structure of the Regulatory Domain of the AraC Family Transcriptional Activator RhaR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03324 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.618, 100.618, 95.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.782 - 2.020 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.19470 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5u93 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.049 | 0.502 |
| Number of reflections | 61172 | |
| <I/σ(I)> | 20.1 | 3 |
| Completeness [%] | 99.6 | 95.8 |
| Redundancy | 6.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES pH 6.0, 50 mM Ca(OAc)2, 12 % PEG 3350 |
