5U96
Crystal structure of the coiled-coil domain from Listeria Innocua (Tetragonal Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 |
| Unit cell lengths | 97.160, 97.160, 52.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.688 - 1.950 |
| R-factor | 0.2382 |
| Rwork | 0.226 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kis |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.557 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.019 | 0.353 |
| Number of reflections | 36018 | |
| <I/σ(I)> | 21.4 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 2.0 M ammonium sulfate, 100 mM HEPES pH 7.5, and 5% glycerol |
