5U8I
DNA Polymerase Beta S229L crystallized in PEG 400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.965, 78.762, 54.651 |
| Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
| Resolution | 37.726 - 2.450 |
| R-factor | 0.1885 |
| Rwork | 0.184 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3isb |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.435 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 5.270 | 2.450 |
| Rmerge | 0.100 | 0.060 | 0.188 |
| Rmeas | 0.108 | ||
| Rpim | 0.040 | ||
| Total number of observations | 95149 | ||
| Number of reflections | 15069 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 91.9 | 99.9 | 76.6 |
| Redundancy | 6.3 | 7.8 | 4 |
| CC(1/2) | 0.999 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 24% PEG 400, 30 mM potassium sodium tartrate, 1% 1,4-dioxane |
