5U7P
Crystal structure of a nucleoside triphosphate diphosphohydrolase (NTPDase) from the legume Trifolium repens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.996, 53.592, 72.835 |
| Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
| Resolution | 24.540 - 1.890 |
| R-factor | 0.18177 |
| Rwork | 0.179 |
| R-free | 0.23541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cji |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.033 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.600 | |
| High resolution limit [Å] | 1.890 | |
| Rmerge | 0.076 | 0.470 |
| Number of reflections | 33604 | |
| <I/σ(I)> | 25.8 | 3.6 |
| Completeness [%] | 99.0 | 96.5 |
| Redundancy | 10.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M ammonium dihydrogen orthophosphate, 15% PEG 3350 |
