5U7F
Co-bound dihydroneopterin triphosphate pyrophosphohydrolase from E. coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.606 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.600, 43.090, 57.230 |
| Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
| Resolution | 57.199 - 1.789 |
| R-factor | 0.1835 |
| Rwork | 0.177 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 201C |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.258 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.199 |
| High resolution limit [Å] | 1.789 |
| Number of reflections | 23041 |
| <I/σ(I)> | 12.61 |
| Completeness [%] | 94.8 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Crystals were grown in 36% PEG 8000, 0.05M Ammonium Sulfate. Crystals were soaked in 0.5mM dihydroneopterin and 5mM CoCl2. |
