5U7E

Apo dihydroneopterin triphosphate pyrophosphohydrolase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2015-11-25
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	53.322, 42.905, 56.953
Unit cell angles	90.00, 91.80, 90.00

Refinement procedure

Resolution	29.071 - 1.942
R-factor	0.1995
Rwork	0.195
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o1c
RMSD bond length	0.002
RMSD bond angle	0.423
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.071
High resolution limit [Å]	1.942
Number of reflections	8908
<I/σ(I)>	12.81
Completeness [%]	92.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	36% PEG 8000, 0.05M Ammonium Sulfate