5U6S

Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and 2-bromobenzoic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.92024
Spacegroup name	P 21 21 21
Unit cell lengths	65.147, 87.249, 162.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.544 - 1.996
R-factor	0.1871
Rwork	0.184
R-free	0.23840
RMSD bond length	0.009
RMSD bond angle	1.042
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.996	2.000
Number of reflections	120959
<I/σ(I)>	38.3	2.3
Completeness [%]	100.0	99.9
Redundancy	9.1	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	22-26 %(w/v) PEG3350, 0.2 M ammonium acetate, 0.1 M MES