5U6N

Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15S), with UDP and salicylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	65.180, 87.562, 163.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.905 - 2.001
R-factor	0.196
Rwork	0.194
R-free	0.24110
RMSD bond length	0.009
RMSD bond angle	1.031
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	63603
<I/σ(I)>	48.6	1.9
Completeness [%]	99.7	99.9
Redundancy	6.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	22-26 % (w/v) PEG3500, 0.2 M ammonium acetate, 0.1 M MES