5U65
Camel Nanobody VHH-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.410, 48.480, 60.890 |
| Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
| Resolution | 55.300 - 2.300 |
| R-factor | 0.20383 |
| Rwork | 0.201 |
| R-free | 0.26052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.834 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.199 | 0.659 |
| Number of reflections | 10575 | |
| <I/σ(I)> | 8.6 | 3.5 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6.4 | 6.6 |
| CC(1/2) | 0.990 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.9M ammonium sulfate, 0.1M bicine |
