5U64
Camel nanobody VHH-28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.790, 76.937, 69.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.395 - 1.153 |
| R-factor | 0.1467 |
| Rwork | 0.145 |
| R-free | 0.17030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.400 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.098 | 1.540 |
| Number of reflections | 53677 | |
| <I/σ(I)> | 17.2 | 1.3 |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 5.9 | 4.3 |
| CC(1/2) | 0.880 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | 0.1M sodium citrate, 0.1M CHES |
