5U4U
pseudoGTPase domain (pG1) of p190RhoGAP-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.937, 62.937, 161.017 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.130 - 1.900 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 15922 | |
| <I/σ(I)> | 11.7 | 1.5 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 8.9 | 7.7 |
| CC(1/2) | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 1.7-1.8 M Lithium Sulfate, 0.1 M N-(2-Acetamido)iminodiacetic acid (ADA) |
