5U3Q
Human PPARdelta ligand-binding domain in complexed with specific agonist 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.460, 94.350, 96.730 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 47.851 - 1.500 |
| R-factor | 0.1596 |
| Rwork | 0.159 |
| R-free | 0.18920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 92879 | |
| <I/σ(I)> | 11.6 | 0.8 |
| Completeness [%] | 83.0 | 28.7 |
| Redundancy | 4.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | Bis-tris propane, potassium chloride, PEG 8000, 1,2-propandiol, EDTA, DTT |
