5U3J
Crystal Structure of DH511.1 Fab in Complex with HIV-1 gp41 MPER Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-20 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.092, 133.963, 179.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.300 - 2.740 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u3p |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.499 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.295 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.133 | 0.800 |
| Number of reflections | 20466 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 6.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 1500 |
