5U36
Crystal Structure Of A Mutant M. Jannashii Tyrosyl-tRNA Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.634, 130.559, 59.956 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 65.280 - 3.030 |
| R-factor | 0.20517 |
| Rwork | 0.201 |
| R-free | 0.28171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pbr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.784 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.280 | 3.100 |
| High resolution limit [Å] | 3.030 | 3.050 |
| Rmerge | 0.850 | |
| Number of reflections | 15184 | |
| <I/σ(I)> | 19.3 | 1.7 |
| Completeness [%] | 98.1 | 95.8 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 15-25% PEG3350, 50 mM sodium cacodylate trihydrate, pH 6.0-7.0 |
