5U32

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	49.944, 56.304, 82.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.487 - 2.191
R-factor	0.1933
Rwork	0.188
R-free	0.24560
Structure solution method	SIRAS
Starting model (for MR)	Hg derivative
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.190	2.190
Rmerge		0.689
Number of reflections	20855	542
<I/σ(I)>	23.9	1.8
Completeness [%]	90.3	85.7
Redundancy	5.3	3.6
CC(1/2)	0.994	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2M sodium dihydrogen phosphate 20% PEG 3350