5U2O
Crystal structure of Zn-binding triple mutant of GH family 9 endoglucanase J30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.960, 90.960, 316.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.510 - 1.460 |
| R-factor | 0.14 |
| Rwork | 0.140 |
| R-free | 0.15100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | J30 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | xia2 |
| Data scaling software | xia2 (0.3.8.0) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV_2447) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.510 | 1.500 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.138 | 1.704 |
| Number of reflections | 134366 | |
| <I/σ(I)> | 17.8 | 2.3 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 21.5 | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | trisodium citrate dihydrate, 2-propanol, PEG 10,000 |
