5U2L
Crystal structure of the Hsp104 N-terminal domain from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.242, 55.242, 109.143 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.974 - 1.655 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20330 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 (v2.0) |
| Data scaling software | HKL-2000 (v2.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.974 |
| High resolution limit [Å] | 1.655 |
| Number of reflections | 23417 |
| <I/σ(I)> | 59.5 |
| Completeness [%] | 99.0 |
| Redundancy | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG3,350 |
