5U24
X-ray structure of the WlaRG aminotransferase from campylobacter jejuni, K184A mutant in complex with TDP-Fuc3N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-04 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.057, 56.795, 124.306 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 28.530 - 1.700 |
| R-factor | 0.1071 |
| Rwork | 0.105 |
| R-free | 0.14627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u1z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.747 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 0.165 |
| Number of reflections | 160895 | |
| <I/σ(I)> | 37.3 | 6 |
| Completeness [%] | 96.6 | 89.4 |
| Redundancy | 3.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS (pH7) 13-17% PEG-5000 10 mM TDP-Fuc3N 1 mM PLP |
