5U20
X-ray structure of the WlaRG aminotransferase from Campylobacter jejuni, internal PLP-aldimine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-10 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.120, 56.621, 124.407 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.14264 |
| Rwork | 0.141 |
| R-free | 0.17751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u1z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.695 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.350 |
| Number of reflections | 235440 | |
| <I/σ(I)> | 9.5 | 1.9 |
| Completeness [%] | 97.4 | 91.6 |
| Redundancy | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS (pH 7) 17-19% PEG-3350 200 mM KCl 1 mM PLP |
