5U1B
Ferritin with Gc MtrE loop2 inserted at the N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 137 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 124.514, 124.514, 314.885 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.890 - 2.810 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bvf |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.653 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.470 | 2.800 |
| Rmerge | 0.166 | 0.069 | 0.796 |
| Number of reflections | 41284 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 93.4 | 100 | 99.9 |
| Redundancy | 4.6 | 4.6 | 4.5 |
| CC(1/2) | 0.984 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | ammonium sulfate, tris |
