5U0R
Crystal Structure of DH270.UCA1 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 209.104, 209.104, 83.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.039 - 3.295 |
| R-factor | 0.2466 |
| Rwork | 0.245 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tyg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.039 | 3.370 |
| High resolution limit [Å] | 3.295 | 3.295 |
| Rmerge | 0.210 | 0.883 |
| Number of reflections | 10410 | |
| <I/σ(I)> | 7 | 1.89 |
| Completeness [%] | 97.6 | 90.45 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1.5M ammonium sulfate and 100mM sodium acetate, pH 4.0 |
