5U0K

C-terminal ankyrin repeats from human liver-type glutaminase (GAB/LGA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 65
Unit cell lengths	85.260, 85.260, 336.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.033 - 2.548
R-factor	0.2202
Rwork	0.218
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u0i
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX ((dev_2733: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.200	84.200	2.640
High resolution limit [Å]	2.550	9.870	2.550
Rmerge	0.094	0.045	0.677
Number of reflections	45005		4413
<I/σ(I)>	14.1
Completeness [%]	99.9	98.6	99.8
Redundancy	8.1	8.5	8.1
CC(1/2)	0.999	0.999	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1.1 M tri-sodium citrate, 0.1 M imidazole, 20 mM glutamine