5U0J

C-terminal ankyrin repeats from human kidney-type glutaminase (KGA) - monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	76.490, 47.180, 115.698
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	46.104 - 1.720
R-factor	0.1854
Rwork	0.183
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u0i
RMSD bond length	0.019
RMSD bond angle	1.454
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX ((dev_2733: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	115.700	63.850	1.750
High resolution limit [Å]	1.720	9.100	1.720
Rmerge	0.056	0.024	0.750
Number of reflections	82659		4303
<I/σ(I)>	15.7
Completeness [%]	94.1	99.1	92.1
Redundancy	6.4	6.9	6.3
CC(1/2)	1.000	1.000	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	3.8 M NH4Cl, 0.1 M Bis-TRIS Propane