5U0I
C-terminal ankyrin repeats from human kidney-type glutaminase (KGA) - tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.290, 75.290, 88.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.088 - 1.423 |
| R-factor | 0.1459 |
| Rwork | 0.144 |
| R-free | 0.17950 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.313 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.290 | 75.290 | 1.450 |
| High resolution limit [Å] | 1.420 | 7.790 | 1.420 |
| Rmerge | 0.121 | 0.029 | 1.458 |
| Rmeas | 0.124 | 0.029 | 1.491 |
| Rpim | 0.026 | 0.006 | 0.309 |
| Total number of observations | 1095048 | 8724 | 54225 |
| Number of reflections | 48262 | 378 | 2342 |
| <I/σ(I)> | 17.1 | 72.8 | 2.9 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 22.7 | 23.1 | 23.2 |
| CC(1/2) | 1.000 | 1.000 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M TRIS, 3.2 M sodium chloride |
