5U0H
Crystal structure of GH family 9 endoglucanase J30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1271 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 91.060, 91.060, 316.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.870 - 1.700 |
R-factor | 0.1414 |
Rwork | 0.141 |
R-free | 0.16570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1CLC |
RMSD bond length | 0.008 |
RMSD bond angle | 0.981 |
Data reduction software | xia2 |
Data scaling software | xia2 (0.3.8.0) |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2447) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 76.520 | 76.520 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.135 | 0.026 | 1.313 |
Rmeas | 0.144 | ||
Rpim | 0.038 | ||
Total number of observations | 1220921 | ||
Number of reflections | 86505 | ||
<I/σ(I)> | 19.3 | 72.9 | 2.2 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 14.1 | 12.2 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | trisodium citrate dihydrate, 2-propanol, PEG 10,000 |