5U01
Cooperative DNA binding by two RelA dimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.910, 117.800, 70.810 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ram |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.730 |
| Number of reflections | 50721 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | Nacit 5.5 100mM, CaCl2 10mM, DTT 10mM, PEG 3350 15%. |
