5U01

Cooperative DNA binding by two RelA dimers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.988
Spacegroup name	P 1 21 1
Unit cell lengths	88.910, 117.800, 70.810
Unit cell angles	90.00, 91.20, 90.00

Refinement procedure

Resolution	29.630 - 2.500
R-factor	0.221
Rwork	0.221
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ram
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.091	0.730
Number of reflections	50721
<I/σ(I)>	13.1	2
Completeness [%]	99.0	99.5
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	Nacit 5.5 100mM, CaCl2 10mM, DTT 10mM, PEG 3350 15%.