5TZ0
Structure of the Fremyella diplosiphon Fluorescence Recovery Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.628, 77.506, 31.968 |
| Unit cell angles | 90.00, 94.15, 90.00 |
Refinement procedure
| Resolution | 38.753 - 3.025 |
| R-factor | 0.2457 |
| Rwork | 0.240 |
| R-free | 0.29220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jdx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.753 | 3.190 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.167 | 1.235 |
| Number of reflections | 5519 | |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.997 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 14% PEG-3000 0.1 M tri-sodium citrate pH 5.5 |
