5TZ0

Structure of the Fremyella diplosiphon Fluorescence Recovery Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	114.628, 77.506, 31.968
Unit cell angles	90.00, 94.15, 90.00

Refinement procedure

Resolution	38.753 - 3.025
R-factor	0.2457
Rwork	0.240
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jdx
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.753	3.190
High resolution limit [Å]	3.020	3.020
Rmerge	0.167	1.235
Number of reflections	5519
<I/σ(I)>	10.7	1.9
Completeness [%]	99.9	99.9
Redundancy	6.8	6.9
CC(1/2)	0.997	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	14% PEG-3000 0.1 M tri-sodium citrate pH 5.5