5TYG

DNA Polymerase Mu Product Complex, 10 mM Mg2+ (960 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-23
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.998, 68.534, 110.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.989 - 1.726
R-factor	0.1562
Rwork	0.155
R-free	0.18530
RMSD bond length	0.007
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.730	4.690	1.730
Rmerge	0.055	0.023	0.909
Number of reflections	48725
<I/σ(I)>	10.1
Completeness [%]	100.0	99.8	100
Redundancy	7.3	6.9	7.3
CC(1/2)		0.999	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000