5TYD

DNA Polymerase Mu Reactant Complex, 10 mM Mg2+ (45 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-23
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.891, 68.590, 110.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.941 - 1.899
R-factor	0.173
Rwork	0.171
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.007
RMSD bond angle	0.898
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.899	5.160	1.900
Rmerge	0.070	0.022	0.963
Number of reflections	36696
<I/σ(I)>	7.4
Completeness [%]	100.0	99.7	100
Redundancy	7.4	6.8	7.4
CC(1/2)		0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000