5TYB

DNA Polymerase Mu Reactant Complex, 10mM Mg2+ (7.5 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-12
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.047, 68.488, 110.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.387 - 1.848
R-factor	0.1688
Rwork	0.167
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.007
RMSD bond angle	0.937
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.083	0.028	0.574
Total number of observations	232161
Number of reflections	39539
<I/σ(I)>	7.4
Completeness [%]	99.6	99.6	95
Redundancy	5.9	5.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000