5TY6
Crystal structure of the broadly neutralizing Influenza A antibody VRC 315 13-1b02 Fab.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.942, 79.055, 107.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.219 - 1.361 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.17260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k9j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.219 | 1.420 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.108 | 0.811 |
| Number of reflections | 106372 | |
| <I/σ(I)> | 8.26 | 1.16 |
| Completeness [%] | 89.8 | 89.3 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.5 M Lithium sulfate, 20% PEG 8000 |
