5TXZ

DNA Polymerase Mu Reactant Complex, 100mM Mg2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-23
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.900, 68.571, 110.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.448 - 1.651
R-factor	0.1674
Rwork	0.166
R-free	0.19400
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.063	0.031	0.712
Rmeas	0.071
Rpim	0.034
Total number of observations	237915
Number of reflections	55422
<I/σ(I)>	9.3
Completeness [%]	100.0	99.6	100
Redundancy	4.3	4	4.3
CC(1/2)		0.998	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000