5TXO

STRUCTURE OF Q151M complex (A62V, V75I, F77L, F116Y, Q151M) mutant HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	104
Detector technology	CCD
Collection date	2013-06-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9181
Spacegroup name	P 1 21 1
Unit cell lengths	89.990, 132.930, 139.120
Unit cell angles	90.00, 97.38, 90.00

Refinement procedure

Resolution	45.989 - 2.546
R-factor	0.192
Rwork	0.191
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v4i
RMSD bond length	0.007
RMSD bond angle	0.956
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.546	2.546
Rmerge	0.091	0.649
Number of reflections	104998
<I/σ(I)>	9.3	1.77
Completeness [%]	99.2	99
Redundancy	3.9	3.3
CC(1/2)		0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE