5TWZ

Structure of Maternal Embryonic Leucine Zipper Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	101.970, 101.970, 62.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.309 - 2.631
R-factor	0.2226
Rwork	0.221
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.747
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.310	2.700
High resolution limit [Å]	2.630	2.630
Rmerge	0.085	1.184
Rmeas	0.093
Rpim	0.036
Total number of observations	75689
Number of reflections	11393
<I/σ(I)>	15.1
Completeness [%]	99.7	99.4
Redundancy	6.6	6.3
CC(1/2)	0.998	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000