5TWP

Pre-catalytic ternary complex of human Polymerase Mu with incoming nonhydrolyzable UMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-09
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.937, 68.696, 110.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.143 - 2.001
R-factor	0.1878
Rwork	0.185
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.008
RMSD bond angle	1.057
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.495
Number of reflections	31042
<I/σ(I)>	14.37	2.71
Completeness [%]	98.8	94.5
Redundancy	6.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	95mM HEPES pH 7.5, 19% PEG4K