5TWG

human MOB1A bound to human MST1 phosphorylated T353 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 43 21 2
Unit cell lengths	60.660, 60.660, 138.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.602 - 2.300
R-factor	0.2172
Rwork	0.215
R-free	0.25240
RMSD bond length	0.013
RMSD bond angle	1.299
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	56.000
High resolution limit [Å]	2.100
Number of reflections	11982
<I/σ(I)>	30
Completeness [%]	96.5
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M MES pH 6.0, 0.2 M LiCl and 20% PEG 6000