5TW1

Crystal structure of a Mycobacterium smegmatis transcription initiation complex with RbpA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	133.012, 161.633, 139.211
Unit cell angles	90.00, 107.72, 90.00

Refinement procedure

Resolution	54.905 - 2.760
R-factor	0.2394
Rwork	0.239
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xlp
RMSD bond length	0.004
RMSD bond angle	0.731
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.859
High resolution limit [Å]	2.760	2.760
Rmerge	0.234	4.816
Number of reflections	143776
<I/σ(I)>	22.39	0.77
Completeness [%]	99.0	100
Redundancy	16.2	12.3
CC(1/2)	0.998	0.214

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M Bis-Tris, pH 6.0, 0.2 M LiSO4, 16% (w/v) polyetheylene glycol 3350, 2.5% (v/v) ethylene glycol