5TVX
Crystal structure of mitochondrial Hsp90 (TRAP1) with ATP in absence of Mg, fully hydrolyzed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 91.4 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.491, 96.820, 125.780 |
Unit cell angles | 90.00, 134.18, 90.00 |
Refinement procedure
Resolution | 39.970 - 2.200 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ipe |
RMSD bond length | 0.007 |
RMSD bond angle | 0.965 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.540 |
High resolution limit [Å] | 1.990 |
Number of reflections | 77629 |
<I/σ(I)> | 7.8 |
Completeness [%] | 99.6 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M NaK Tartrate, 20% (v/v) PEG3350, 36 mM hexamine cobalt, 5 mM ATP, 5 mM EDTA |