5TVW
Crystal structure of mitochondrial Hsp90 (TRAP1) with ATP in absence of Mg, hemi-hydrolyzed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 91.4 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.490, 97.181, 125.904 |
| Unit cell angles | 90.00, 134.64, 90.00 |
Refinement procedure
| Resolution | 39.894 - 2.500 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ipe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.595 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.670 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.043 | |
| Number of reflections | 98531 | 8330 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M NaK Tartrate, 20% (v/v) PEG3350, 36 mM hexamine cobalt, 5 mM ATP, 5 mM EDTA |
