5TVQ
Mouse Tdp2 catalytic domain bound to SUMO2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 64 |
| Unit cell lengths | 106.838, 106.838, 56.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.748 - 2.350 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gz1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 40.000 | 1.810 |
| High resolution limit [Å] | 2.350 | 3.770 | 1.750 |
| Rmerge | 0.056 | 0.034 | 0.509 |
| Rmeas | 0.061 | 0.039 | 0.581 |
| Rpim | 0.024 | 0.018 | 0.275 |
| Total number of observations | 110520 | ||
| Number of reflections | 15578 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 99.5 | 98.8 | 100 |
| Redundancy | 7.1 | 4.3 | 4.3 |
| CC(1/2) | 0.998 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM MES, 18% PEG 8000, 200 mM calcium acetate |
