5TUU

Crystal structure of the E2F4-DP1 coiled coil and marked-box domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	I 1 2 1
Unit cell lengths	73.575, 37.550, 109.942
Unit cell angles	90.00, 103.44, 90.00

Refinement procedure

Resolution	53.466 - 2.251
R-factor	0.2072
Rwork	0.205
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2AZE
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.960	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.056	0.310
Number of reflections	13407
<I/σ(I)>	20.3	2.9
Completeness [%]	94.0	93.6
Redundancy	3.5	2.4
CC(1/2)	0.997	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES pH 6.5, 15% PEG 6000