5TUP

X-ray Crystal Structure of the Aspergillus fumigatus Sliding Clamp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	56.950, 103.370, 79.200
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	44.024 - 2.602
R-factor	0.199
Rwork	0.196
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cs5
RMSD bond length	0.003
RMSD bond angle	0.752
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.680	78.680	2.720
High resolution limit [Å]	2.600	9.010	2.600
Rmerge	0.189	0.065	2.018
Rmeas	0.204
Rpim	0.076
Total number of observations	197392
Number of reflections	27932
<I/σ(I)>	7.2
Completeness [%]	99.5	98.7	96.7
Redundancy	7.1	6.5	6.6
CC(1/2)	0.994	0.995	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	0.2 M KCl, 20 % PEG 3350