5TUG

Archaellum periplasmic stator protein complex FlaF and FlaG from Sulfolobus acidocaldarius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9918
Spacegroup name	P 61
Unit cell lengths	119.919, 119.919, 152.403
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.152 - 2.470
R-factor	0.1653
Rwork	0.163
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p94
RMSD bond length	0.008
RMSD bond angle	0.985
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.152	2.560
High resolution limit [Å]	2.470	2.470
Rmerge	0.101	1.291
Number of reflections	44562
<I/σ(I)>	19.2	2.4
Completeness [%]	100.0	100
Redundancy	11.4	11.5
CC(1/2)	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	18% PEG 6000 0.1M Tris, pH 7.5, 0.2M NaBr